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Image Search Results
Journal: Diagnostic Pathology
Article Title: Quality assessment of a clinical next-generation sequencing melanoma panel within the Italian Melanoma Intergroup (IMI)
doi: 10.1186/s13000-020-01052-5
Figure Lengend Snippet: Clinical characteristics of the metastatic melanoma patients
Article Snippet: All specimens had already been screened for the presence of BRAF codon 15 mutations by SS approach and Real Time PCR assay (
Techniques: Mutagenesis
Journal: Diagnostic Pathology
Article Title: Quality assessment of a clinical next-generation sequencing melanoma panel within the Italian Melanoma Intergroup (IMI)
doi: 10.1186/s13000-020-01052-5
Figure Lengend Snippet: Primer sequences and PCR amplification conditions for Sanger Sequencing (SS) validation
Article Snippet: All specimens had already been screened for the presence of BRAF codon 15 mutations by SS approach and Real Time PCR assay (
Techniques: Amplification, Sequencing
Journal: Diagnostic Pathology
Article Title: Quality assessment of a clinical next-generation sequencing melanoma panel within the Italian Melanoma Intergroup (IMI)
doi: 10.1186/s13000-020-01052-5
Figure Lengend Snippet: Variants called by the three NGS systems with a coverage of at least 200x and a VAF ≥10%
Article Snippet: All specimens had already been screened for the presence of BRAF codon 15 mutations by SS approach and Real Time PCR assay (
Techniques:
Journal: Journal of Comparative Effectiveness Research
Article Title: Variant analysis of SARS-CoV-2 strains with phylogenetic analysis and the Coronavirus Antiviral and Resistance Database
doi: 10.2217/cer-2021-0208
Figure Lengend Snippet: The neighbor-joining tree construction method and Jukes-Cantor nucleotide distance measures were carried out with other sequences from all reference lineages from GISAID using CLC Sequence Viewer 8.0 (Qiagen, Aarhus A/S, Denmark) software. Bootstrap values are arranged as 1000 replicates. Because of numerous samples, the phylogenetic tree has been constructed as circular and rooted. The GISAID accession numbers of SARS-CoV-2 lineages are: alpha B.1.1.7-1 (EPI_ISL_3098706), alpha B.1.1.7-2 (EPI_ISL_3098709), delta B.1.617.1-1 (EPI_ISL_ 3066844), delta B.1.617.1-2 (EPI_ISL_ 3066436), delta B.1.617.2-1 (EPI_ISL_3098714), delta B.1.617.2-2 (EPI_ISL_3098716), beta B.1.351-1 (EPI_ISL_3098444), beta B.1.351-2 (EPI_ISL_3098450), eta B.1.525-1 (EPI_ISL_3089259), eta B.1.525-2 (EPI_ISL_3089260), gamma P.1-1 (EPI_ISL_3091694), gamma P.1-2 (EPI_ISL_3092082), lota B.1.526-1 (EPI_ISL_3092079), lota B.1.526-2 (EPI_ISL_3098714., WT: Wild type (EPI_ISL_3049390). The GenBank accession number of SARS-CoV-2 Wuhan-Hu-1 isolate: MN908947.3.
Article Snippet: Two variant-specific screening PCR kits (
Techniques: Sequencing, Software, Construct
Journal: Journal of Comparative Effectiveness Research
Article Title: Variant analysis of SARS-CoV-2 strains with phylogenetic analysis and the Coronavirus Antiviral and Resistance Database
doi: 10.2217/cer-2021-0208
Figure Lengend Snippet: The distribution of distinct and missense mutations using phylogenetic analysis and the Coronavirus Antiviral and Resistance Database.
Article Snippet: Two variant-specific screening PCR kits (
Techniques: Mutagenesis